Comparison of DNA polymerases for quantification of single nucleotide differences by primer extension assays.

en, depending on the rotisserie motor kit you choose. Widely available power bars can serve as an on/off switch for motors not equipped with their own. Membranes are inserted into the 50 mL tubes. The surface carrying the nucleic acids or proteins should face inwards. Membranes should be moist to prevent breakage and air bubble formation from surface tension. To start the operation, reagent solution is added to the tube, which is then capped and inserted into a grip clip for incubation. Regular procedures for western, northern, northwestern or Southern blotting, including washing, can be used with this apparatus. This apparatus saves time, mess, expensive reagents and can substitute for relatively expensive equipment at room temperature or 65°C inside a dry oven. Results are comparable to those obtained with a commercial rotisserie apparatus.